(36)Chromatographic Separation of the Fatty Acids.

Separation of the higher fatty acids.

Previous papers from this laboratory (1, 2) reported the development of systems in which the normal saturated fatty acids up to Cl8 had partition ratios sufficiently different from one another to permit their separation by counter-current distribution. However, the quantitative aspect of the distribution of the Cl0 to Cl8 acids was impaired by the occurrence of skewing. It appeared desirable to...

Separation of Free Fatty Acids using Supercritical Fluid Chromatography

The separation of free fatty acids was performed with supercritical fluid chromatography (SFC) on analytical lab scale apparatus. Experiments were performed using fatty acids, which occur in vegetable oils in different amounts. Free fatty acids were analyzed without derivatization. Several stationary phases of packed columns and types of detectors were used to achieve efficient free fatty acids...

Separation of a protein factor necessary for the oxidative desaturation of fatty acids in the rat.

Microsomes from rat liver were extracted by low ionic strength solutions. Extracted microsomes lost most of the linoleic acid desaturation activity. The addition of the extract back into the extracted microsomes was necessary to restore full desaturation activity. The soluble fraction had no desaturation activity. The existence of a soluble factor loosely bound to the microsomes, stable to soni...

Determination of Tocopherols and Fatty Acids in Seeds of

  Background: The dried fruits of Silybum marianum (L.) Gaertn (Compositae) contain silymarin, an isomeric mixture of flavonolignans. Silymarin acts as a strong anti -hepatotoxic. This fruits contains a high amount of oil .  Objective: The objective of this study was to determine total lipid content, fatty acid composition and content of different kind of tocopherols in seed oils of S. marianum...

The separation of the C12-C18 fatty acids by reversed-phase partition chromatography.